qPCR & PCR
Polymerase chain reaction (PCR) is a core and widely used laboratory method. An enhancement of this method, qPCR (quantitative PCR) measures the amplification of DNA in real time and not at the end of cycling like conventional PCR. Together these applications have contributed to significant advances in gene expression, genotyping, cloning, and other applications.
Central to PCR experimental success is the design and high quality synthesis of primers or probes. Primers and probes that are prematurely truncated, contain incorrect base sequences, or include carryover contaminants from manufacturing processes can impede accurate and efficient PCR.
Over several decades, we have refined a proprietary platform that improved nucleotide coupling efficiency during oligo synthesis. Subsequent purification steps ensure a high quality primer or probe for consistent performance. All oligos are QC tested in-house by ESI mass spectroscopy to ensure an accurate, full length, final product. Moreover, years of experience with nucleotide chemistries and thermodynamics has been incorporated into advanced algorithms powering our easy-to-use sequence design tools.
Differences and changes in gene expression are important measures to understand biological systems. One of the most widely used methods of analyzing gene expression is quantitative PCR (qPCR) following cDNA synthesis. qPCR, also called real-time PCR, uses a fluorescent reporter molecule with a primer pair to measure target nucleic acid amplification during PCR cycling.
We offer a selection of qPCR products to assess gene expression. Order a ready-to-use solution with PrimeTime® probe-based assays and master mix, or create a completely custom option from our probe catalog.
Using commercially produced master mixes, enzymes, and reagents ensures simple, efficient experiment setup and consistent data. Each batch of reagents from Labspec undergoes rigorous quality assessments for performance and reproducibility, moving you one step closer to achieving successful and consistent qPCR results.
The accuracy of design and synthesis of a primer pair is the most important consideration to generate good PCR performance data. Poor design choices, erroneous or truncated sequences, and ineffective purification can lead to unusable results. We have applied thermodynamic and bioinformatic knowledge towards a suite of easy-to-use, online tools to help you design primers. We also enable consistent, quality data by providing primers in a variety of formats that have been synthesized on our state-of-the-art oligonucleotide synthesis platforms.
qPCR probe design and production quality are paramount in creating specific qPCR assays with robust signal-to-noise performance. Labspec qPCR probes are HPLC purified to ensure removal of free residual dye and truncated synthesis products that can contribute to high background signal.
Good probe design must balance the need for high affinity with considerations for best signal generation and quenching. We have a variety of options to address even complex design requirements. Generate high quality performance data with PrimeTime® or our other probes by leveraging our state-of-the-art oligonucleotide manufacturing, proprietary quenchers, and choice of license-free dyes.
Researchers often look for genetic variations to better understand phenotypic traits, such as plant fruit production or human disease incidence. One individual’s genome may differ from others in numerous ways, including single nucleotide polymorphisms (SNPs), insertions or deletions (indels), or copy number variations (CNV).
Polymerase chain reaction (PCR) is a commonly used genotyping technique. Often employing a primer-pair and target-specific fluorescent probe, quantitative PCR (qPCR) can be a sensitive and specific way to detect SNPs. Labspec offers a complete SNP-typing solution with predesigned assays, as well as complementary easy- and ready-to-use reagent mixes. For other applications, modified probes are available that can be incorporated into custom qPCR assay.
Products for in vitro diagnostics (IVDs) and laboratory-developed tests
Our GMP services place an emphasis on making you and your requirements the top priority while also demonstrating an expertise and commitment to enabling discoveries in biology and medicine. When you work with our team, a single dedicated account advocate will provide you with personalized and consistent management of your GMP experience, from project feasibility through commercial or clinical production. We ensure that your products are made to your exact specifications while adhering to your requirements at every step.
- Easily transition from RUO to IVD by aligning specifications and methods from research to GMP
- Receive a full range of OEM services, including customized formulation, labeling, and packaging
- Obtain license-free fluorophores, including fluorescein, Cy®, rhodamine, and ATTO™ dyes