Next Generation Sequencing(NGS)

Next generation sequencing (NGS) technologies are rapidly becoming an integral part of genetic research and discovery. The ability to generate large amounts of sequence data in a relatively short amount of time is helping to enable a wide range of genetic analysis applications and accelerate advances in research, clinical and applied markets.

Critical to the success of obtaining high quality results in NGS experiments is the availability of high quality tools to effectively prepare fragment libraries representing the original nucleic acid samples. From platform adapters to our portfolio of xGen ® products for targeted sequencing, Labspec’s mission is to deliver innovative, high performing NGS products to enable you to increase your discovery power.

Adapters for next generation sequencing

High quality adapters that meet the ever-expanding needs of researchers using NGS

Adapters are a key component of the next generation sequencing (NGS) workflow. Whether your project requires a basic adapter or a sophisticated design for higher accuracy, IDT has the products and expertise to deliver the right solution.

Regardless of the NGS instrument or application, Labspec has been serving the needs of NGS scientists for over 10 years. We are recognized as the leader in adapter synthesis based on our experience in custom oligo manufacturing and our commitment to quality. Additionally, our NGS customers and partners benefit from:

  • A comprehensive adapter offering
  • Innovative designs
  • Trusted customer support
  • Complete customizability

rhAmpSeq amplicon sequencing system—NGS power. PCR simplicity.

Faster discovery with a rapid and accurate targeted sequencing solution

The rhAmpSeq system enables highly accurate amplicon sequencing on Illumina® next generation sequencing (NGS) platforms. Whether you are investigating thousands of targets or a few, the fast and easy rhAmpSeq workflow, based on our proprietary RNase H2-dependent PCR (rhAmp PCR) technology, generates NGS-ready amplicon libraries for deep, targeted resequencing.

  • Achieve cost-effective library preparation using custom or predesigned panels
  • Reduce the formation of primer dimers or misprimed PCR products
  • Perform only 2 PCR amplification steps in a fast, easy workflow
  • Ensure robust results with IDT manufacturing quality

Harnessing the power of rhAmp PCR

Our proprietary rhAmp PCR technology drives the rhAmpSeq system. Using RNA-base–containing blocked primers (rhAmp primers), this technology harnesses the intrinsic properties of the RNase H2 enzyme to recognize and cleave DNA:RNA duplexes. rhAmp PCR is thus a powerful tool for increasing amplification specificity and minimizing the biggest limitation of PCR multiplexing—primer dimers.

The rhAmpSeq system combines the performance advantages of rhAmp PCR with a fast, easy-to-use workflow that requires only 2 PCR amplification steps (Figure 1) to generate amplicon libraries for Illumina sequencing platforms. Figure 1 shows both amplification steps in the rhAmpSeq workflow, highlighting the role of rhAmp PCR technology during the first amplification step (Targeted rhAmp PCR 1). Coupled with our best-in-class DNA and RNA manufacturing, the rhAmpSeq system offers high quality, targeted, sequencing results quickly and cost-effectively.