DNA & RNA Purification & Analysis
Our nucleic acid purification products are optimized to provide maximum yield, purity, and integrity from virtually any sample type. Explore our Invitrogen product line and other Invitrogen technology choices for efficient purification of RNA, miRNA, plasmid DNA, and gDNA. Learn about our innovative Invitrogen Cells-to-CT products for bypassing nucleic acid isolation altogether, as well as new kits to work with FPPE samples.
Cells constitute discrete units of biological function and serve as starting points in a myriad of studies to identify and map many of the basic biochemical and physical processes of life. Compiling data sets from multiple cell analysis investigations allows scientists to better understand, predict, and—ultimately—influence the factors that underlie cell health, proliferation, function, and death.
We offer a broad range of cell analysis research solutions including Gibco cell culture media and reagents, Invitrogen labeling and detection technologies, antibodies and immunoassays, Invitrogen EVOS cell imaging systems, Invitrogen Countess II FL Automated Cell Counter, and the Invitrogen Attune Nxt Flow Acoustic Focusing Cytometer.
PCR (polymerase chain reaction) is an invaluable tool for molecular biology research. It is used daily in laboratories around the world in a wide array of applications such as cloning, gene expression analysis, genotyping, sequencing, and mutagenesis. We offer products to help you achieve PCR success for virtually any application. With trusted Applied Biosystems thermal cyclers and real-time PCR systems, industry-leading Invitrogen SuperScript and Platinum Taq enzymes, and millions of reliable Applied Biosystems TaqMan Assays, you’ll find everything you need to help you achieve PCR success.
Comprehensive sequencing solutions
Choose from gold-standard Sanger sequencing technology and fragment analysis on the capillary electrophoresis (CE) platform to flexible and scalable next-generation sequencing (NGS) to help your clinical research lab realize its full sequencing potential.
Choosing a Cloning Method
A number of different methods, technologies, and protocols are available for performing the actual cloning reaction. To choose the one that best meets your needs, you will need to analyze the current parameters of your experiment and plan ahead for any downstream procedures. This section discusses several highly efficient cloning technologies:
- Restriction Enzyme Digestion & Ligation Cloning: is an industry standard for molecular biologists
- TOPO Cloning: provides simple, convenient reactions typically in less than 5 min
- Gateway Cloning Technology offers the greatest flexibility in vector choices and downstream applications
- Seamless Cloning and Assembly: Tools optimized to clone up to 4 DNA fragments simultaneously into virtually any linearized E. coli vector in a 30 minute room temperature reaction
- Type IIs Assembly: Clone up to 8 fragments simultaneously and without scars, and avoid rearrangements when cloning homologous or repetitive sequences
- GeneArt Gene Synthesis and Strings DNA Fragments: 100% accuracy and optimization of genes to maximize expression
Detect, measure, and analyze protein expression, identity, and function
Researchers look to us for innovative, high-quality products, which include our Invitrogen and Thermo Scientific Pierce reagents, devices, and kits: Novex protein gels, SuperSignal chemiluminescent substrates, BCA and other protein assays, ELISA kits, Slide-A-Lyzer dialysis cassettes, cell lysis reagents; mass spectrometry sample prep and quantitation reagents, western blotting components and instruments, and crosslinking and labeling reagents. Learn about protein analysis techniques and find the research tools and reagents you need from our vast portfolio of products.