Laboratory Stains
CYTOCHROME STAIN WITH BUFFER
Staining for Blood cells and Hemoparasites.
PRINCIPLE:
Blood smears are prepared and fixed (using the Fixative). The cellular components when stained turn blue (basophilic) or orange granules (acidophilic). The color intensity can be varied by adjusting the staining time.
KIT COMPONENTS:
Reagent bottles.
Kit insert.
PHYSICAL APPEARANCE:
Cytochrome stain clear ink blue coloured liquid without any particles.
Cytochrome buffer clear colourless liquid without any particles.
PACK SIZE
2 x 50 ml
4 x 50 ml
4 x 250 ml
STORAGE TEMPERATURE:
Room Temperature.
SHELF LIFE:
60 months.
SIGNS OF DETERIORATION:
1. Colour change.
2. Growth.
AVAILABILITY:
In Stock.
DISPATCH TIME IN DAYS:
1 to 2 days.
KIT DIMENSIONS:
2 x 50ml – 85x105x45 mm.
4 x 50ml – 170x105x45 mm.
4 x 250ml – 125x140X 125mm.
APPROXIMATE WEIGHT OF KIT:
2 x 50 ml – 266 gms.
4 x 50 ml – 493 gms.
4 x 250ml – 1.1 kg.
NO OF KITS IN SHIPPER:
2 x 50 ml – 92 kits.
4 x 50 ml – 46 kits.
4 x250 ml – 12 kits.
DIMENSION OF SHIPPER:
410x550x378 mm
APPROXIMATE WEIGHT OF SHIPPER:
2 x 50 ml – 26 kg.
4 x 50 ml – 24 kg.
4 x250 ml – 15 kg.
SAMPLES AVAILABILITY:
Yes.
PAPANICOLAOU STAIN
Study of morphology of squamous epithelial cell
Principle:
Papanicolau Stains are used in conjunction with Hematoxylin nuclear stains in the diagnosis of malignant cytological disease. EA-36 and EA-50 are used in conjunction with OG-6 for gynecological staining. EA-65 is used with OG-6 for non-gynecological staining. When performed properly, the stained specimen should display hues from the entire spectrum: red, orange, yellow, green, blue, and violet. The chromatin patterns are well visible, the cells from borderline lesions are easier to interpret and the photomicrographs are better. The staining results in very transparent cells, so even thicker specimens with overlapping cells can be interpreted.
Kit components:
Reagent bottles
Kit insert.
Physical Appearance:
Hematoxylin clear wine red coloured liquid without any particles.
OG-6 clear orange color liquid without any particles.
Light green SF green color clear liquid with reddish purple tinge without any particles.
Dehydrant colorless liquid without any particles.
Xylene colorless liquid without any particles.
Ana fix colorless liquid without any particles.
Pack Size:
250 SMEARS
PROCEDURE:
1. Prepare a smear on clear, dry glass slide.
2. Fix with Ana fix.
3. Pour Dehydrant Reagent on slide for 3 minutes.
4. Wash with distilled water.
5. Flood the smear with Hematoxylin reagent for 1 min.
6. Add 3 drops of Phosphate Buffer which is not provided for 10 seconds.
7. Wash with Dehydrant 2 times for 30 seconds.
8. Pour Working Stain on slide. Allow it to remain for 45 seconds.
9. Wash with Dehydrant for 30 seconds.
10. Rinse with Xylene.
11. Allow the slide to air dry.
12. Mount with glass cover using a drop of DPX.
RESULTS:
When Staining with Papanicolaou cytoplasm appears with various shades of blue, pink, orange red and green.
Wright’s Stain With Buffer
Principle:
Blood smears are prepared and fixed (using the Fixative). The cellular components when stained turn blue (basophilic) or orange granules (acidophilic). The color intensity can be varied by adjusting the staining time.
Kit components:
Reagent bottles
Kit insert.
Physical Appearance:
Wright stain: Clear ink blue coloured liquid without any particles.
Wright stain buffer: Clear colourless liquid without any particles
Malaria Parasite Stain
Test for differential staining of cells for detection of parasites by Red and Blue stain.
Principle:
Blood smears are prepared and fixed (using the Fixative). The cellular components when stained turn blue (basophilic) or orange granules (acidophilic). The color intensity can be varied by adjusting the staining time.
Kit components:
Reagent bottles
Kit insert.
Physical Appearance:
Blue stain clear blue coloured liquid without any particles.
Red stain clear amber liquid without any particles
NEISSER′S METHYLENE BLUE
Staining Solution for Metachromatic staining.
Principle:
Well-developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractile bodies within the bacterial cytoplasm. With basic dye, they tend to stain more strongly than the rest of the bacterium, and with toluidine blue or methylene blue they stain metachromatically, a reddish purple colour. They are demonstrated most clearly by special methods, such as Albert’s and Neisser’s, which stain them dark purple but the remainder of the bacterium with a contrasting counterstain. The diphtheria bacillus gives its characteristic volutin-staining reactions best in a young culture (18-24 hours) on a blood or serum medium.
Kit components:
Reagent bottles
Kit insert.
Physical Appearance:
Neissers Methylene blue clear blue coloured liquid without any particles.
Pack Size:
1 x 100ml
1 x 500 ml
Storage Temperature:
Room Temperature.
Shelf life:
60 months
Signs of Deterioration
1. Colour change
2. Growth
Availability:
In Stock.
GIEMSA STAIN (READY TO USE)
Test for differential staining of cells for detection of parasites by Giemsa stain.
PRINCIPLE:
Smears are fixed using the Giemsa Fixative. Slides are immersed in Giemsa Reagent to differentially stain specific cellular components. The cellular components stain either basophilic (blue) or eosinophilic (orange granules). The color intensity can be varied by adjusting the staining time.
KIT COMPONENTS:
Reagent bottles.
Kit insert.
PHYSICAL APPEARANCE:
Giemsa stain clear purple coloured liquid without any particles.
PACK SIZE:
1 x 100ml
1 x 500 ml
STORAGE TEMPERATURE:
Room Temperature.
SHELF LIFE:
60 months
SIGNS OF DETERIORATION:
1.Colour change
2.Growth
AVAILABILITY:
In Stock.
GIEMSA STAIN WITH BUFFER
Test for differential staining of cells for detection of parasites by Giemsa stain.
PRINCIPLE:
Smears are fixed using the Giemsa Fixative. Slides are immersed in Giemsa Reagent to differentially stain specific cellular components. The cellular components stain either basophilic (blue) or eosinophilic (orange granules). The color intensity can be varied by adjusting the staining time.
KIT COMPONENTS:
Reagent bottles.
Kit insert.
PHYSICAL APPEARANCE:
Giemsa stain clear ink blue coloured liquid without any particles
Giemsa buffer clear colourless liquid without any particles
PACK SIZE:
2 x 50 ml
4 x 50 ml
4 x 250 ml
STORAGE TEMPERATURE:
Room Temperature.
SHELF LIFE:
60 months
SIGNS OF DETERIORATION:
1.Colour change
2.Growth
AVAILABILITY:
In Stock
JSB I&II Stain Kit
Intended Use:
Staining for Blood cells and Hemoparasites and Malaria Parasites.
Principal:
Blood smears are prepared and fixed (using the Fixative). The cellular components when stained turn blue (basophilic) or orange granules (acidophilic). The color intensity can be varied by adjusting the staining time.
Specification
| Physical Appearance | JSB I clear blue colored liquid without any particles. JSB II clear amber liquid without any particles |
| Kit components | Reagent bottles. Kit insert |
| Pack Size | 2 x 50 ml 4 x 50 ml 4 x 250 ml |
| Storage Temperature | Room Temperature |
| Dispatch time in weeks | 2 to 4 weeks |
| Kit dimensions | 2 x 50ml – 85x105x45 mm 4 x 50ml- 170x105x45 mm 4 x 250ml – 125x140x125mm |
| Supply Capacity | 600 mn ml per annum |
May Grunwald Stain
Intended Use:
Staining for Blood cells and Hemoparasites.
Principal:
Blood smears are prepared and fixed (using the Fixative). The cellular components when stained turn blue (basophilic) or orange granules (acidophilic). The color intensity can be varied by adjusting the staining time.
Specification
| Physical Appearance | May Grunwald’s stain clear violet coloured liquid without any particles. Giemsa stain clear violet blue liquid without any particles. Giemsa stain buffer clear colourless liquid without any particles. |
| Kit components | Reagent bottles. Kit insert |
| Pack Size | 3 x 50 ml 3 x 250 ml |
| Storage Temperature | Room Temperature |
| Dispatch time in weeks | 2 to 4 weeks |
| Kit dimensions | 3 x 50ml – 170x105x45 mm 3 x 250ml – 125x140X 125mm |
| Supply Capacity | 600 mn ml per annum |
H&E STAIN
Intended Use:
HISTOPATHOLOGY STAIN.
Principal:
Alum acts as mordant and hematoxylin containing alum stains the nucleus light blue. This turns red in presence of acid, as differentiation is achieved by treating the tissue with acid solution. Bluing step converts the initial soluble red color within the nucleus to an insoluble blue color. The counterstaining is done by using eosin which imparts pink color to the cytoplasm.
Specification
| Physical Appearance | Hematoxylin clear wine rad colored liquid without any particles. Eosin clear reddish orange liquid without any particles. Dehyrant clear colorless liquid without any particles. |
| Kit components | Reagent bottles. Kit insert |
| Pack Size | 4 x 50 ml 4 x 250 ml |
| Storage Temperature | Room Temperature |
| Shelf Life | 60 MOnths |
| Kit dimensions | 4 x 50ml- 170x105x45 mm 4 x 250ml – 125x140x125mm |
| Dispatch Time In Weeks | 2 to 4 weeks. |
| Supply Capacity | 600 mn ml per annum |