5x HOT FIREPol® SolisGreen qPCR Mix
Suitable for ROX-dependent and ROX-independent qPCR cyclers
For in vitro use only
HOT FIREPol® SolisGreen qPCR Mix is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
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