5x HOT FIREPol® Blend Master Mix Ready to Load

with 10 mM MgCl2

For in vitro use only



5x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.


HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase. 


In addition, the mix also allows amplification of longer fragments.


Generated PCR products are compatible with blunt-end and TA cloning procedures (to increase the blunt end cloning efficiency treat the PCR products with T4 DNA polymerase or DNA polymerase I large Klenow fragment prior to cloning).

Sample size: 0.1 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.